Tamara Mocking

Postdoc, LACDR

Tamara Mocking is a postdoctoral researcher at Leiden Academic center for drug research (LACDR), Leiden university.  She studied biopharmaceutical sciences (Bsc & Msc) at Leiden university and obtained her PhD working on modulation of GPCR signaling using light sensitive-molecules containing a photoswitchable moiety as tools to influence GPCR activity with light at the Vrije Universiteit (VU) Amsterdam in the group of Prof dr. Rob Leurs. Currently, she a postdoc in the group of Prof. Dr. Laura Heitman where her main focus is to aid in the development of novel label free assays to study Solute Carrier proteins (SLCs). This family of transporter proteins are currently underappreciated due to a lack of available tools to study these membrane proteins. However, these SLC have a great potential as novel therapeutic targets, as they facilitate transport of a large variety of molecules ( i.e. drugs, neurotransmitters, hormones, ions ect.) across the cell membrane and, thereby regulate cellular homeostasis. Within the ReSOLUTE consortium we aim to enhance SLC research by developing novel tools and assays to study and deorphanize this class of transporter proteins.

Presentation: Label-free detection of prostaglandin transporter (SLCO2A1) activity using a TRACT assay

The prostaglandin transporter (PGT, SLCO2A1) mediates transport of prostanoids (a.o. prostaglandin E2 (PGE2)) into the cells to promote their degradation. Overexpression of PGT leads to low extracellular PGE2 levels and has been linked to impaired wound healing of diabetic foot ulcers. Inhibition of PGT would be beneficial, however, there are currently no high-through screening assays for this transporter.  Here we developed a label-free impedance-based assay for PGT that measures transport activity through receptor activation (TRACT).Uptake of PGE2 by PGT will reduce extracellular PGE¬2 levels and thereby attenuated the response of co-expressed EP3 or EP4 receptor. Thus, PGT activity is detected as a change in receptor activity. Induction of PGT expression on EP3 or EP4 expressing HEK293-JumpIn-SLCO2A1 cells results in over 10-fold reduction in potency of PGE2. Potency was recovered upon inhibition of the PGT-mediated PGE2 uptake with PGT inhibitor Olmesartan and T26A. The results confirm that prostanoid receptor activity can be used as a measure of PGT activity. Conclusion: An impedance-based TRACT assay was established that measures prostaglandin transporter (SLCO2A1) activity through prostanoid receptor signaling. This will enable a novel way to better study the wound healing capacity of SLCO2A1 inhibition on a cellular level.

Contact           

Connect                   

Veerstraat 27
1211 HJ Hilversum

Chamber of Commerce: 32110979
VAT no: NL8184.34.491.B01
         
 
 

                                    


Register now

© Copyright 2023 by Hyphen Projects | All rights reserved